Transferrin-binding proteins of Neisseria meningitidis
نویسندگان
چکیده
منابع مشابه
Acquisition of heme iron by Neisseria meningitidis does not involve meningococcal transferrin-binding proteins.
Similarities in size between hemin-binding protein 1 (HmBP1) and transferrin-binding protein 1 (TBP1) of Neisseria meningitidis suggest that these proteins are functionally homologous. However, a meningococcal mutant lacking the transferrin-binding proteins retained the capacity to acquire iron from heme and hemoglobin. In immunoblots, hyperimmune polyclonal antiserum against TBP1 did not react...
متن کاملMolecular Docking Analysis of Human Transferrin and Lactoferrin Binding Proteins of Neisseria Meningitidis with 5 Hydroxy 1-4 Naphthoqinone
Neisseria meningitidis is the principal cause of bacterial meningitis.. Bacteria can be transmitted by carriers through the droplets of respiratory secretions and can only infect humans. The mortality rate of bacterial meningitis is very high even after the discovery of many antibiotics. Acquisition of iron is the major determinant in the pathogenesis of Neisseria meningitidis which is fulfille...
متن کاملTransferrin-binding protein B of Neisseria meningitidis: sequence-based identification of the transferrin-Binding site confirmed by site-directed mutagenesis.
A sequence-based prediction method was employed to identify three ligand-binding domains in transferrin-binding protein B (TbpB) of Neisseria meningitidis strain B16B6. Site-directed mutagenesis of residues located in these domains has led to the identification of two domains, amino acids 53 to 57 and 240 to 245, which are involved in binding to human transferrin (htf). These two domains are co...
متن کاملRecombinant Neisseria meningitidis transferrin binding protein A protects against experimental meningococcal infection.
To better characterize the vaccine potential of Neisseria meningitidis transferrin binding proteins (Tbps), we have overexpressed TbpA and TbpB from Neisseria meningitidis isolate K454 in Escherichia coli. The ability to bind human transferrin was retained by both recombinant proteins, enabling purification by affinity chromotography. The recombinant Tbps were evaluated individually and in comb...
متن کاملThe 70 kilodalton iron regulated protein of Neisseria meningitidis is not the human transferrin receptor.
Neisseria meningitidis is able to chelate iron from human transferrin (HTF), the main sequestrator of extracellular iron in vivo. Previous workers have reported that a ca. 70 kilodalton (kDa) iron regulated outer membrane protein (FeRP-70) is a highly specific receptor for HTF. We have examined the interaction between the iron regulated outer membrane proteins (OMP's) and HTF, using HTF and rab...
متن کامل